Journal:

Article Title: Cell Surface Collagenolysis Requires Homodimerization of the Membrane-bound Collagenase MT1-MMP

doi: 10.1091/mbc.E06-08-0740

Figure Lengend Snippet: Expression of MT1F-ΔCat and MT1F-ΔCatΔTM inhibits tubule formation of MDCK cells in type I collagen gel. (A) MDCK cells stably transfected with the plasmids for MT1F-ΔCat, MT1F-ΔCatΔTM, or empty vector were analyzed by Western blot using anti-FLAG M2 antibody. Sup, culture supernatant accumulated for 24 h; Cell, cell lysates. Arrows indicate the bands of recombinant proteins. *Degradation products. (B) Transfected MDCK cells were cultured in type I collagen gel (2 mg/ml) with or without hepatocyte growth factor (50 ng/ml). Bright field images were captured on the 7th day of the culture. Bar, 200 μm.

Article Snippet: Cell Culture and Transfection COS7, HT1080, and MDCK cells were cultured in DMEM (BioWhittaker, Walkersville, MD) supplemented with 10% FBS and penicillin/streptomycin (BioWhittaker).

Techniques: Expressing, Stable Transfection, Transfection, Plasmid Preparation, Western Blot, Recombinant, Cell Culture